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KMID : 0381120080300040319
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Genes and Genomics
2008 Volume.30 No. 4 p.319 ~ p.327
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Enhanced Tolerance Against Osmotic Stresses of Escherichia coli Cells Expressing Soybean KS-Type Dehydrin
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Chung Eun-Sook
Cho Chang-Woo
Kim Kee-Young
Kim Kyung-Mee
So Hyun-Ah
Lee Seon-Woo
Lee Young-Choon
Kim Kyoung-Sook
Chung Young-Soo
Lee Jai-Heon
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Abstract
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Two low temperature induced genes designated as SLTI66 and SLTI629 encoding KS-type dehydrin were cloned from soybean (Glycine max L.) by suppression subtractive hybridization. RNA expression of SLTI66 and SLTI629 gene was abundantly induced by low temperature, salt, dehydration stress and transiently induced by heat stress and abscisic acid. E. coli cells expressing SLTI66 and SLTI629 protein showed dramatic resistance to freezing stress compared to the control cells. The cells expressing SLTI629 exhibited resistance to salt stress compared to the cells containing the vector or the SLTI66 expression construct. We propose that SLTI66 and SLTI629 play a different role as a protector against osmotic stresses.
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KEYWORD
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abscisic acid, dehydrin, osmotic stress, RNA expression, tolerance
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